Predicting transcriptional outcomes of novel multigene perturbations with GEARS.
实验目的：Understanding cellular responses to genetic perturbation is central to numerous biomedical application, from identifying genetic interations involved in cancer to developing methods for regenerative medicine.
挑战： The combinatorial explosion in the number of possible multigene perturbations severely limits experimental interrogation.
GEARS: graph-enhanced gene activation and repression simulator. A method that integrates deep learning with a knowledge graph of gene-gene relationships to predict transcriptional responses to both single and multigene perturbations using single-cell RNA-sequencing data from perturbational screens.
这段话不是很理解：The screens were run using the Perturb-seq assay, which combines a pooled screen with a single-cell RNA-sequencing readout of the entire transcriptome for each cell. 什么是 Perturb-seq assay，什么又是 pooled screen
“pooled screen” 指的是把多个扰动一起进行实验，而不是一个一个单独做，节省时间和资源。
